enzyme lined immunosorbent assay elisa kit  (R&D Systems)

Journal: BioMed research international

Article Title: Agmatine protects against zymosan-induced acute lung injury in mice by inhibiting NF-κB-mediated inflammatory response.

doi: 10.1155/2014/583736

Figure Lengend Snippet: Figure 5: Agmatine inhibits Zymosan-induced NF-𝜅B activation and DNA binding activity in lung. 6 h after ZYM injection with or without agmatine treatments, mice were exsanguinated and their lungs were removed. (a) Western blot was performed to detect p-I𝜅B-𝛼and I𝜅B-𝛼 in cytoplasm and NF-𝜅B p65 in nucleus. Expressions of 𝛽-actin and LaminB1 were shown as loading controls. (b) DNA binding activity of NF-𝜅B p65 was examined by a TransAM p65 transcription factor ELISA kit. Each bar represents the mean ± SD of 8 mice. ∗𝑃< 0.05 compared with NS group. #𝑃< 0.05 compared with Zymosan group.

Article Snippet: Mouse TNF-α, IL-1β, and IL-6 enzyme-lined immunosorbent assay (ELISA) kits were purchased from Boster Biotechnology (Wuhan, China).

Techniques: Activation Assay, Binding Assay, Activity Assay, Injection, Western Blot, Enzyme-linked Immunosorbent Assay